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anti mouse rat rage antibody  (R&D Systems)


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    Structured Review

    R&D Systems anti mouse rat rage antibody
    Anti Mouse Rat Rage Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 97 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+rage/Mouse%2FRat+RAGE+Antibody/pm42014719-313-4-7
    Average 95 stars, based on 97 article reviews
    anti mouse rat rage antibody - by Bioz Stars, 2026-10
    95/100 stars

    Images

    Related Articles

    Control:

    Article Title: Cyclophilin A is a ligand for RAGE in thrombo-inflammation.
    Article Snippet: . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Cyclophilin A is a ligand for RAGE in thrombo-inflammation

    Recombinant:

    Article Title: Cyclophilin A is a ligand for RAGE in thrombo-inflammation.
    Article Snippet: . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Cyclophilin A is a ligand for RAGE in thrombo-inflammation

    Shear:

    Article Title: Cyclophilin A is a ligand for RAGE in thrombo-inflammation.
    Article Snippet: . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Cyclophilin A is a ligand for RAGE in thrombo-inflammation

    Immunohistochemistry:

    Article Title: Alveolar epithelial paxillin in postnatal lung alveolar development
    Article Snippet: Anti-pro SPC (AB3786; 1:100 for IHC) and -β-actin (A5441; 1:1000 for IB) antibodies were from Sigma (St. Louis, MO, USA). .. Anti-RAGE (MAB1179; 1:100 for IHC) antibody was from R&D systems (Minneapolis, MN, USA). .. The retroviral WZLneo-C/EBPa was a gift from Kai Ge (Addgene Plasmid, 34567).

    Article Title: Alveolar epithelial paxillin in postnatal lung alveolar development.
    Article Snippet: Anti-pro SPC (AB3786; 1:100 for IHC) and -β-actin (A5441; 1:1000 for IB) antibodies were from Sigma (St. Louis, MO, USA). .. Anti-RAGE (MAB1179; 1:100 for IHC) antibody was from R&D systems (Minneapolis, MN, USA). .. Molecular biological and biochemical methods The retroviral WZLneo-C/EBPa was a gift from Kai Ge (Addgene Plasmid, 34567).

    Flow Cytometry:

    Article Title: RAGE deficiency ameliorates autoimmune hepatitis involving inhibition of IL-6 production via suppressing protein Arid5a in mice.
    Article Snippet: As for the surface staining, first the cells were stained with Fixable Viability Stain 510(BD Biosciences Cat# 564406, RRID:AB_2869572), then blocked with CD16/32(BioLegend Cat# 101320, RRID:AB_1574975) antibody and then stained with surface antibody (CD45(BioLegend Cat# 103115, RRID:AB_312980;Cat# 103131, RRID:AB_893344), CD3(BioLegend Cat# 100206, RRID:AB_312663), CD4(BD Biosciences Cat# 562891, RRID:AB_2737870), CD8(BioLegend Cat# 100714, RRID:AB_312753), NK1.1(BioLegend Cat# 108706, RRID:AB_313393), CD69(BioLegend Cat# 104513, RRID:AB_492844)) for 30 min at 4 °C, and finally washed with PBS. .. For detection of RAGE by flow cytometry, anti-RAGE (R&D Systems Cat# MAB11795, RRID:AB_10971764) were first stained and then secondary antibodies were stained. .. As for intracellular staining, the cells were stimulated with RPMI 1640 medium containing 10% FBS and Cell Activation Cocktail (with Brefeldin A) (1:500, BioLegend) for 5 h. After stimulation, the cells were stained with surface markers (CD45(BioLegend Cat# 103131, RRID:AB_893344), CD3(BioLegend Cat# 100203, RRID:AB_312660), CD4(BioLegend Cat# 100412, RRID:AB_312697),CD8(BioLegend Cat# 100714, RRID:AB_312753), CD11C(BioLegend Cat# 117327, RRID:AB_2129642) and F4/80(BioLegend Cat# 123108, RRID:AB_893502)) for 30 min at 4 °C.

    Staining:

    Article Title: RAGE deficiency ameliorates autoimmune hepatitis involving inhibition of IL-6 production via suppressing protein Arid5a in mice.
    Article Snippet: As for the surface staining, first the cells were stained with Fixable Viability Stain 510(BD Biosciences Cat# 564406, RRID:AB_2869572), then blocked with CD16/32(BioLegend Cat# 101320, RRID:AB_1574975) antibody and then stained with surface antibody (CD45(BioLegend Cat# 103115, RRID:AB_312980;Cat# 103131, RRID:AB_893344), CD3(BioLegend Cat# 100206, RRID:AB_312663), CD4(BD Biosciences Cat# 562891, RRID:AB_2737870), CD8(BioLegend Cat# 100714, RRID:AB_312753), NK1.1(BioLegend Cat# 108706, RRID:AB_313393), CD69(BioLegend Cat# 104513, RRID:AB_492844)) for 30 min at 4 °C, and finally washed with PBS. .. For detection of RAGE by flow cytometry, anti-RAGE (R&D Systems Cat# MAB11795, RRID:AB_10971764) were first stained and then secondary antibodies were stained. .. As for intracellular staining, the cells were stimulated with RPMI 1640 medium containing 10% FBS and Cell Activation Cocktail (with Brefeldin A) (1:500, BioLegend) for 5 h. After stimulation, the cells were stained with surface markers (CD45(BioLegend Cat# 103131, RRID:AB_893344), CD3(BioLegend Cat# 100203, RRID:AB_312660), CD4(BioLegend Cat# 100412, RRID:AB_312697),CD8(BioLegend Cat# 100714, RRID:AB_312753), CD11C(BioLegend Cat# 117327, RRID:AB_2129642) and F4/80(BioLegend Cat# 123108, RRID:AB_893502)) for 30 min at 4 °C.

    Bioprocessing:

    Article Title: Klebsiella pneumoniae infection induces an S100A8/A9-mediated autocrine loop in human airway epithelium to amplify inflammation
    Article Snippet: Endotoxin-free S100A8/A9 heterodimer (Calprotectin) was purchased from R&D Systems (Cat# 2220-S8-050). .. The following function-blocking monoclonal antibodies or western blotting antibodies were used: anti-TLR4 (InvivoGen Cat# mabg-htlr4-2) and anti-RAGE (R&D Systems, Cat # 1145-RG). .. Anti-total IκBα (Cat# 4814) and β -actin (Cat# 4970) were purchased from Cell Signaling Technology.

    Western Blot:

    Article Title: Klebsiella pneumoniae infection induces an S100A8/A9-mediated autocrine loop in human airway epithelium to amplify inflammation
    Article Snippet: Endotoxin-free S100A8/A9 heterodimer (Calprotectin) was purchased from R&D Systems (Cat# 2220-S8-050). .. The following function-blocking monoclonal antibodies or western blotting antibodies were used: anti-TLR4 (InvivoGen Cat# mabg-htlr4-2) and anti-RAGE (R&D Systems, Cat # 1145-RG). .. Anti-total IκBα (Cat# 4814) and β -actin (Cat# 4970) were purchased from Cell Signaling Technology.

    Immunostaining:

    Article Title: A novel leptin receptor binding peptide tethered-collagen scaffold promotes lung injury repair.
    Article Snippet: Lung regeneration after acute injury usually depends on stem cell migration and differentiation, and functional alveoli-like tissue and capillary structure formation.. The homing of mesenchymal stem cells (MSCs) to injury sites promotes lung repair through damaged cell replacement and anti-inflammatory and anti-fibrotic effects.. Here, we aimed to improve therapeutic effects of the endogenous MSCs by increasing their homing efficiency.

    other:

    Article Title: Alveolar epithelial progenitor cells require Nkx2-1 to maintain progenitor-specific epigenomic state during lung homeostasis and regeneration
    Article Snippet: anti-GFP (Rabbit, Abcam, ab290 – 1:1000) TSA Plus Fluorescein (Akoya Biosciences, NEL741001KT) anti-RAGE (Rat, R&D Systems, MAB1179 – 1:100) anti-Sox2 (Rabbit, Seven Hills Bioreagents, Rb1236 – 1:200) anti-Sox9 (Rabbit, Sigma Aldrich, AB5535 – 1:100) anti-Cdx2 (Rabbit, Cell Marque, EPR2764Y – 1:500) anti-Gata4 (Rabbit, Santa Cruz, sc-9053 – 1:200) anti-Pdx1 (Goat, Abcam, ab47383 – 1:5000) anti-Krt8 (Rat, DSHB, TROMA-I – 1:200) TSA Plus Cyanine 3.5 (Akoya Biosciences, NEL763001KT anti-Sftpc (Rabbit, Seven Hills Bioreagents, Rb09337 – 1:250) anti-Ki67 (Rabbit, Abcam, ab16667 [SP6] – 1:100) TSA Plus Cyanine 3.5 (Akoya Biosciences, NEL763001KT) TSA Plus Cyanine 5 (Akoya Biosciences, NEL745001KT) anti-E-Cadherin (Rat, R&D Systems, MAB7481 – 1:1000) anti-TTF1/Nkx2-1 (Rabbit, Seven Hills Bioreagents, Rb1231 – 1:1000) TSA Plus Fluorescein (Akoya Biosciences, NEL741001KT) TSA Plus Cyanine 5 (Akoya Biosciences, NEL745001KT)



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    Immunoproteasome is required for aged AT2 cell regenerative decline and the establishment of IFN γ+ T cells in tertiary lymphoid structures. A) Schematic figure to show the culture of alveolar organoids from young or aged immunoproteasome knockout (IP KO) and control C57BL/6 (WT) mice in feeder-free media at 7 days of growth, including B) organoid forming efficiency (OFE) and (C) diameter. D) RTqPCR of the IFNγ-inducible MHC-I complex component B2m in isolated lung AT2 cells from young or aged immunoproteasome KO and WT mice. E) Representative immunofluorescence staining of Ki-67 to mark proliferating cells with AT2 cell marker SPC and AT1 cell <t>marker</t> <t>Rage/Ager</t> in corresponding feeder-free organoids from aged mice in A-C. F) The ratio of Ki-67+ proliferating cells in aged immunoproteasome knockout (IP KO) versus WT mice. G) Dysregulated pathways in aged immunoproteasome KO epithelium in comparison to WT based on DEG genes (padj < 0.05, LogFC <0), with bar size representing enrichment score. H) Flow cytometry quantifications of CD8+ and CD4+ (I) T cells as % of all CD45+ immune cells in aged immunoproteasome KO and WT control lungs. J) Zoomed-in multiplex fluorescent images of TLS in aged lungs of immunoproteasome KO and WT controls with K) corresponding absolute quantification of CD8+ T cell and IFNγ+ cell (L) densities. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
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    Image Search Results


    Immunoproteasome is required for aged AT2 cell regenerative decline and the establishment of IFN γ+ T cells in tertiary lymphoid structures. A) Schematic figure to show the culture of alveolar organoids from young or aged immunoproteasome knockout (IP KO) and control C57BL/6 (WT) mice in feeder-free media at 7 days of growth, including B) organoid forming efficiency (OFE) and (C) diameter. D) RTqPCR of the IFNγ-inducible MHC-I complex component B2m in isolated lung AT2 cells from young or aged immunoproteasome KO and WT mice. E) Representative immunofluorescence staining of Ki-67 to mark proliferating cells with AT2 cell marker SPC and AT1 cell marker Rage/Ager in corresponding feeder-free organoids from aged mice in A-C. F) The ratio of Ki-67+ proliferating cells in aged immunoproteasome knockout (IP KO) versus WT mice. G) Dysregulated pathways in aged immunoproteasome KO epithelium in comparison to WT based on DEG genes (padj < 0.05, LogFC <0), with bar size representing enrichment score. H) Flow cytometry quantifications of CD8+ and CD4+ (I) T cells as % of all CD45+ immune cells in aged immunoproteasome KO and WT control lungs. J) Zoomed-in multiplex fluorescent images of TLS in aged lungs of immunoproteasome KO and WT controls with K) corresponding absolute quantification of CD8+ T cell and IFNγ+ cell (L) densities. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

    Journal: bioRxiv

    Article Title: Local IFNγ signaling contributes to the regenerative decline of aged alveolar progenitor cells

    doi: 10.64898/2026.04.07.716929

    Figure Lengend Snippet: Immunoproteasome is required for aged AT2 cell regenerative decline and the establishment of IFN γ+ T cells in tertiary lymphoid structures. A) Schematic figure to show the culture of alveolar organoids from young or aged immunoproteasome knockout (IP KO) and control C57BL/6 (WT) mice in feeder-free media at 7 days of growth, including B) organoid forming efficiency (OFE) and (C) diameter. D) RTqPCR of the IFNγ-inducible MHC-I complex component B2m in isolated lung AT2 cells from young or aged immunoproteasome KO and WT mice. E) Representative immunofluorescence staining of Ki-67 to mark proliferating cells with AT2 cell marker SPC and AT1 cell marker Rage/Ager in corresponding feeder-free organoids from aged mice in A-C. F) The ratio of Ki-67+ proliferating cells in aged immunoproteasome knockout (IP KO) versus WT mice. G) Dysregulated pathways in aged immunoproteasome KO epithelium in comparison to WT based on DEG genes (padj < 0.05, LogFC <0), with bar size representing enrichment score. H) Flow cytometry quantifications of CD8+ and CD4+ (I) T cells as % of all CD45+ immune cells in aged immunoproteasome KO and WT control lungs. J) Zoomed-in multiplex fluorescent images of TLS in aged lungs of immunoproteasome KO and WT controls with K) corresponding absolute quantification of CD8+ T cell and IFNγ+ cell (L) densities. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

    Article Snippet: We used the following primary antibodies: α-SPC (1:500, AB3786, Millipore), α-RAGE/AGER (1:500, MAB1179-100, R&D System), α-Ki-67 (1:200, 550609, BD Bioscience).

    Techniques: Knock-Out, Control, Isolation, Immunofluorescence, Staining, Marker, Comparison, Flow Cytometry, Multiplex Assay, Quantitative Proteomics